Real-Time PCR

Nanogen MGB Alert™ Real-Time PCR Reagents Use Minor Groove Binder (MGB) Technology.

Nanogen has developed a proprietary MGB moiety that provides optimal performance for DNA probe-target hybridization reactions. By attaching the MGB moiety to the 3'-end or the 5'- end of a DNA probe during synthesis on a commercial synthesizer or post-synthetically to an amine modified oligo, the MGB moiety folds back into the minor groove formed by the DNA duplex to stabilize hybridization.

The effect of this stabilization is an increase in melting temperature and the MGB moiety produces a "Tm leveling" effect as A-T content increases.

The increase in the melting temperature due to the presence of the MGB moiety allows the use of shorter probes with improved mismatch discrimination.

Advantages of Nanogen’s MGB real-time PCR technology:

• Use of short, or highly conserved, specific sequence effects
• Specific primers and non cleavable probes
• Post PCR melt curve analysis
• Novel Nanogen nucleotides reduce sequence effects
• Ability to distinguish genetic variants